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目的 测定特异性细胞毒 T淋巴细胞 (CTL )对胃癌细胞的体外杀伤活性及观察形态学改变。方法 用分离胃腺癌患者的癌细胞经丝裂霉素 C处理后 ,作为抗原与抗CD3单克隆抗体、重组白细胞介素 - 2刺激患者自体外周血单个核细胞 ,体外诱导 CTL,用 MTT比色法测定 CTL 对自体癌细胞、人胃癌细胞株 (BGC- 82 3和 MGC- 80 3)的体外杀伤活性。将 CTL与 BGC- 82 3共同温育 ,通过透射和扫描电镜观察杀伤靶细胞的形态改变。结果 CTL对自体胃癌细胞有特异性杀伤作用 ,可使靶细胞凋亡或溶解坏死。结论 体外混合细胞培养诱导出的 CTL,对靶细胞有明显的杀伤作用
Objective To determine the cytotoxic activity of specific cytotoxic T lymphocytes (CTL) on gastric cancer cells in vitro and observe their morphological changes. METHODS: After mitomycin C was used to treat cancer cells from patients with gastric adenocarcinoma, the autologous peripheral blood mononuclear cells were stimulated with anti-CD3 monoclonal antibody and recombinant interleukin-2 to induce CTL in vitro. The in vitro cytotoxic activity of CTL on autologous cancer cells and human gastric cancer cell lines (BGC-82 3 and MGC-803) was measured. The CTLs were co-incubated with BGC-82 3 , and the morphological changes of the killer target cells were observed by transmission and scanning electron microscopy. RESULTS: CTL had specific killing effect on autologous gastric cancer cells, which could cause apoptosis or lysis and necrosis of target cells. Conclusion CTL induced by mixed cell culture in vitro has obvious killing effect on target cells