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动物实验表明人体器官内淋巴郁滞可引起许多疾病或使其恶化。本研究采用0.05ml~(125)I标记的人血浆r球蛋白(5g/dL,185KBq,5μCi)兔肝被膜下注射,电子计算机数字闪烁出现两种清除参量,快速清除参量与血流对同位素的吸收有关,慢速清除参量与淋巴循环和蛋白分解有关。在正常对照组11只兔中,慢速清除平均半清除时为288分钟(标准误24.7)。在10只胸导管结扎的兔中,24小时后肝脏发生中度水肿,慢速清除参量显示半清除时延迟到平均525分,与正常对照组相比,有极为显著的统计学意义(P<0.001,标准误19.1)。然而也说明,胸导管虽然结扎,仍有部分淋巴液回流。胸导管结扎所引起的这种中度淋巴郁滞的实验模型,与人体许多疾病所引起的淋巴郁滞相类似。在另外9只兔也行胸导管结扎,并在结扎后立即腹腔注射香豆素(25mg/kg)。这种药物可以从组织中通过蛋白的分解作用移除过多的蛋白质。24小时后同样方法测定,本组动物的半清除时为286分(标准误12.8)。与正常对照组相比,没有差异性。但是与没有用药的胸导管结扎组相比,却有非常显著的统计学意义(P<0.001)。本实验结果提示放射性同位素的示踪研究,可应用于器官内淋巴的循环测定,同时证实了香豆素对肝脏及其它内脏器官有关疾病的治疗作用。
Animal experiments show that lymphoid disorders in human organs can cause or worsen many diseases. In this study, rabbit liver was injected subcutaneously with 0.05ml 125I labeled human plasma globulin (5g / dL, 185KBq, 5μCi). Two numbers of scintigraphy were removed by electronic computer scintillation, Absorption related to slow clearance parameters and lymphatic circulation and proteolysis. Of the 11 rabbits in the normal control group, the average removal time was 288 minutes (standard error 24.7) with a slow clearance. In 10 thoracic duct ligation rabbits, moderate edema was observed in the liver 24 hours later, and the slow clearance parameters showed an average delay of 525 points in half clearance, which was significantly different from the normal control group (P < 0.001, standard error 19.1). However, it also shows that although the thoracic duct ligation, there are still some lymph reflux. This experimental model of moderate lymphadenopathy caused by thoracic duct ligation resembles lymphatic stasis caused by many diseases in the body. Thoracic catheters were also ligated in another 9 rabbits and coumarin (25 mg / kg) was injected intraperitoneally immediately after ligation. The drug removes too much protein from tissues through the breakdown of the protein. 24 hours after the same method was measured, the group of animals when the half-cleared to 286 points (standard error of 12.8). Compared with the normal control group, there is no difference. However, there was a statistically significant (P <0.001) comparison with the non-dosed thoracic duct ligation group. The results of this experiment suggest that the radioisotope tracer study can be applied to the determination of the circulation of the endolymphatic organs, and at the same time, the therapeutic effect of coumarin on the diseases of the liver and other internal organs is confirmed.