本实验采用T细胞有丝分裂原刀蛋白A(ConA)在体外刺激正常小鼠脾细胞,并用rIL-2扩增培养,获得了具有MHC非限制性杀瘤活性的杀伤细胞(ConA-LAK),探讨了其杀瘤机制,主要结果如下:(1)先用ConA刺激预培养24小时的小鼠脾细胞,然后加用rlL-2,可诱导小鼠淋巴细胞转化增殖.(2)ConA-LAK介导MHC非限制性杀瘤活性,可杀伤YAC-1(NK敏感),L929(TNF敏感)和MHC-1类抗原阴性的 In this experiment, splenocytes from normal mice were stimulated with T cell mitogen-inducing protein A (ConA) and expanded with rIL-2 to obtain killer cells (ConA-LAK) with a non-selective killing activity of MHC. The main results are as follows: (1) The splenocytes of mice pre-incubated with ConA for 24 hours are stimulated first, and then rlL-2 is added to induce lymphocyte transformation and proliferation in mice. (2) ConA-LAK Unrestricted anti-tumor activity of MHC, killing YAC-1 (NK sensitive), L929 (TNF sensitive) and MHC-1 antigen negative