Objective: We study the adhesion moleclues on the surface of SO-Rb50 retinoblastoma cell line.Motheds : The distribution of proteoglycans in the retinoblastoma SO-Rb50 cell line was analyzed by histochem-electron microscopy, using Colloidal Iron in combination with a series of enzyme digestions. In addition, immunohistochemistry was performed using a panel of specific antibodies including neuron specific enolase(NSE) ,glial fibrillary acidic protein(GFAP) , S-100 protein, fibronectin, laminin, and collagen IV.Results: Immunohistochemical stains showed the most marked cytoplasmic reactivity of SO-Rb50 cells with anti-NSE and anti-S100. The cells member and surface was postive with anti-NSE. No reactivity was noted with antibodies against laminin, GFAP, and collagen IV. After incubated with colloidal iron solution, three types of colloidal iron-positive stained material could be distinguished based on difiierencens in shape, size, electron density: (1) electron dense particles, (2) the larger colloidal ir Objective: We studied the adhesion molecules on the surface of SO-Rb50 retinoblastoma cell line. Motheds: The distribution of proteoglycans in the retinoblastoma SO-Rb50 cell line was analyzed by histochem-electron microscopy, using Colloidal Iron in combination with a series of enzyme digestions. In addition, immunohistochemistry was performed using a panel of specific antibodies including neuron specific enolase (NSE), glial fibrillary acidic protein (GFAP), S-100 protein, fibronectin, laminin, and collagen IV. Results: Immunohistochemical stains showed the most marked cytoplasmic reactivity of SO-Rb50 cells with anti-NSE and anti-S100. The cells member and surface was postive with anti-NSE. No reactivity was noted with antibodies against laminin, GFAP, and collagen IV. After incubated with colloidal iron solution , three types of colloidal iron-positive stained material could be distinguished based on difierences in shape, size, electron density: (1) electron dense particles, (2) the l arger colloidal ir