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不同浓度的卡那霉素结合羧苄青霉素、头孢霉素对沙田柚子叶、上胚轴、下胚轴的分化及新梢生长的影响试验结果表明：培养基中卡那霉素浓度超过５０ｍｇ／Ｌ时，不利于诱导产生愈伤组织，也不利于上、下胚轴形成不定芽；卡那霉素浓度大于７５ｍｇ／Ｌ时，明显抑制愈伤组织的分化和新梢的生长；羧苄青霉素及头孢霉素浓度为３００—４００ｍｇ／Ｌ时抑菌效果较好，对外植体的生长分化影响亦较小。
Effects of different concentrations of kanamycin combined with carbenicillin and cefotaxime on the differentiation and shoot growth of grapefruit leaves, hypocotyl and hypocotyls of Shatian showed that the concentration of kanamycin in the medium exceeded 50mg / L, it is not conducive to the induction of callus formation, is not conducive to the formation of adventitious buds on the hypocotyl; kanamycin concentration greater than 75mg / L, significantly inhibited callus differentiation and shoot growth; carbenicillin When the concentration of cefotaxime was 300-400mg / L, the antibacterial effect was better and the growth and differentiation of the explants were less affected.