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目的研究趋化因子CCL18对前列腺肿瘤细胞侵袭力、迁移力和细胞增殖的影响。方法通过培养前列腺肿瘤细胞LNCa P、DU145,并加入趋化因子蛋白CCL18,利用Transwell实验、Wound Healing实验和CCK8试剂盒来分别检测CCL18对前列腺肿瘤细胞侵袭力、迁移力及对细胞增殖的影响。结果经过趋化因子蛋白CCL18处理的前列腺肿瘤细胞,在Transwell实验中,肿瘤细胞跨膜细胞数显著增加(LNCa P:对照vs.CCL18=202.0±18.5 vs.279.7±27.6;DU145:对照vs.CCL18=60.3±6.5 vs.91.0±9.5);Wound Healing实验中,肿瘤细胞发生迁移的数量明显增加(LNCa P:对照vs.CCL18=127.3±14.3 vs.214.4±30.9;DU145:对照vs.CCL18=68.6±15.8 vs.129.4±12.0);细胞生长实验结果揭示肿瘤细胞增殖速度也显著加快。结论趋化因子蛋白CCL18能促进前列腺肿瘤细胞LNCa P、DU145的侵袭力、转移力,并加快前列腺肿瘤细胞的生长速度。
Objective To investigate the effect of chemokine CCL18 on invasion, migration and cell proliferation of prostate cancer cells. Methods The effects of CCL18 on invasiveness, migration and cell proliferation of prostate cancer cells were detected by Transwell assay, Wound Healing assay and CCK8 kit by culturing prostate tumor cells LNCa P, DU145 and chemokine CCL18. Results Prostate tumor cells treated with chemokine protein CCL18 showed a significant increase in the number of transmembrane cells in the Transwell assay (LNCa P: control vs. CCL18 = 202.0 ± 18.5 vs. 279.7 ± 27.6; DU145: control vs. CCL18 = 60.3 ± 6.5 vs.91.0 ± 9.5). In Wound Healing, the number of tumor cells migrating significantly increased (LNCa P: control vs. CCL18 = 127.3 ± 14.3 vs..214.4 ± 30.9; DU145: control vs. CCL18 = 68.6 ± 15.8 vs.129.4 ± 12.0). The results of cell growth assay also showed that the proliferation of tumor cells was significantly accelerated. Conclusion The chemokine CCL18 can promote the invasiveness and metastatic potential of prostate cancer cells LNCa P and DU145 and accelerate the growth of prostate tumor cells.