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为了观察肺泡巨噬细胞(AM)对肺表面活性物质(PS)分泌的影响,本实验以激活的AM培养上清液对离体非灌流肺进行持续灌洗,观察肺内PS含量的变化。结果表明,由调理酵母多糖(OPZ)激活的AM上清液,能使肺磷脂释放量(PL)和肺磷脂释放指数(b)增大,与激活物(OPZ)对照组及细胞对照组比较,有显著差异,P<0.01。用消炎痛预处理AM 30min后,再用OPZ激活AM,PL值和b值减少;而用细胞松弛素B预处理AM30min后,再用OPZ激活AM,则PL值和b值增大。以上两组与对照组比较,P<0.01。这表明激活AM增强肺PS分泌的作用能被消炎痛所抑制,而被细胞松弛素所增强,提示激活AM增强肺PS分泌可能是以前列腺素为中介的。激活AM能使PS分泌增加,这对于在生理和病理情况下稳定肺泡表面张力和增强肺的防御机能有重要意义。
In order to observe the effect of alveolar macrophages (AM) on the secretion of pulmonary surfactant (PS), in vitro cultured non-perfusion lungs were continuously lavaged with activated AM supernatant to observe the change of PS content in the lung. The results showed that the release of pulmonary lecithin (PL) and the release of pulmonary phlebitis index (b) increased with the AM supernatant activated by OPZ, compared with the control group and the cell-control group , There was a significant difference, P <0.01. After pretreatment with indomethacin for 30 min, AM was further activated by OPZ, and the values of PL and b were decreased. After pretreated with cytochalasin B for 30 min and then activated by OPZ, the values of PL and b increased. The above two groups compared with the control group, P <0.01. This suggests that the activation of AM to enhance the secretion of pulmonary PS can be inhibited by indomethacin and enhanced by cytochalasin, suggesting that activation of AM to enhance pulmonary PS secretion may be prostaglandin-mediated. Activation of AM increases PS secretion, which is important for stabilizing alveolar surface tension and enhancing lung defenses under physiological and pathological conditions.