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目的研究中药川续断和丹参在大鼠正畸牙移动过程中对其牙周组织改建的作用,并比较两种不同中药在改建过程中的异同。方法选取72只8周龄SPF级Wistar雌性大鼠,建立大鼠正畸牙移动实验模型,随机分为川续断组、丹参组和对照组3组,每组24只,川续断组每日灌服6g/kg川续断水煎剂,丹参组每日灌服6g/kg丹参水煎剂,对照组每日灌服3mL生理盐水,每隔7d加力1次。3组动物于正畸加力7、14、21、28d分批次处死,每批次6只。剥离头颅骨,分别测量牙移动的距离及牙槽骨的密度,同时制作上颌第一磨牙区牙周组织切片,光学、电子显微镜观察牙周组织改建情况,并进行统计学分析。结果川续断组、丹参组牙移动距离差异无统计学意义(P>0.05),但是二者均大于对照组,差异有统计学意义(P<0.05);光镜下观察3组破骨细胞数均呈现先增加后平缓趋势,川续断组、丹参组比对照组增加更为显著(P<0.05),川续断组和丹参组之间差异无统计学意义(P>0.05)。3组牙槽骨密度呈现降低趋势,川续断组、丹参组比对照组降低缓慢(P<0.05),川续断组和丹参组之间差异无统计学意义(P>0.05)。结论灌服丹参和川续断水煎剂均促进大鼠正畸牙移动过程中破骨细胞的增殖和分化,加快牙槽骨吸收及其修复重建,有利于正畸牙移动。
Objective To study the effect of Chuanxiong broken and salvia miltiorrhiza on the reconstruction of periodontal tissue during orthodontic tooth movement in rats and to compare the similarities and differences between the two kinds of traditional Chinese medicines during their remodeling. Methods Seventy-two Wistar female rats of 8 weeks old were used to establish the experimental model of orthodontic tooth movement in rats. They were randomly divided into three groups: Chuanxuetuan group, Danshen group and control group, 24 rats in each group. Diltiazem 6g / kg Dipsacus decoction, Salvia group daily dosing 6g / kg Danshen decoction, the control group daily drench with 3mL saline, every 7d force 1 time. Three groups of animals were sacrificed in batches on the 7th, 14th, 21st and 28th days after orthodontic force, 6 in each batch. The skull was stripped, the distance of tooth movement and the density of alveolar bone were measured, and the periodontal tissue sections of maxillary first molar area were made. The reconstruction of periodontal tissue was observed by optical and electron microscopes, and statistical analysis was made. Results There was no significant difference in tooth movement distance between Chuanxuetuan group and Salvia miltiorrhiza group (P> 0.05), but the difference was statistically significant (P <0.05). Three groups of osteoclasts (P <0.05). There was no significant difference between Chuanxuetuan group and Salvia miltiorrhiza group (P> 0.05). The density of alveolar bone in the three groups showed a decreasing trend. The Chuanxuetuan group and the Salvia miltiorrhiza group were lower than those in the control group (P <0.05). There was no significant difference between the Chuanxiong group and the Salvia miltiorrhiza group (P> 0.05). Conclusion Radix Salviae Miltiorrhizae and Chuan Xiu Duan decoction can promote the proliferation and differentiation of osteoclasts during orthodontic tooth movement and accelerate the alveolar bone resorption and repair, which is propitious to orthodontic tooth movement.