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目的初步了解新疆和甘肃结核分枝杆菌的基因型、主要流行型及其分布特点,比较两省区之间基因型分布。方法采用随机抽样的方法挑选新疆地区结核分枝杆菌临床分离株179株,甘肃地区结核分枝杆菌临床分离株176株,采用间隔寡核苷酸分型(Spoligotyping)技术对所收集的临床分离株进行分型研究,分析我国西北部相邻的两省区结核分枝杆菌的基因型分布、主要流行型及其地区特征。结果新疆地区菌株分为47个基因型,其中28个基因型为新的型别,新疆菌株中北京家族占68.72%(123/179),其次为T家族(6.70%,12/179),H家族(3.91%,7/179)和CAS家族(2.23%,4/179);甘肃地区菌株分为29种基因型,其中10个基因型为新的型别,甘肃菌株中北京家族占86.93%(153/176),其次为T家族(4.55%,8/176),H家族(1.14%,2/176),MANU2(1.14%,2/176)和CAS(0.57%,1/176)。结论北京基因型菌株在新疆和甘肃地区均为主要流行株,但甘肃地区北京基因型菌株比例明显高于新疆地区。
Objective To understand the genotype, prevalence and distribution of Mycobacterium tuberculosis in Xinjiang and Gansu provinces and to compare the distribution of genotypes between the two provinces. Methods A total of 179 strains of clinical isolates of Mycobacterium tuberculosis from Xinjiang and 176 strains of clinical isolates of Mycobacterium tuberculosis from Gansu province were selected by random sampling. Spoligotyping was used to detect clinical isolates of clinical isolates The genotyping, main epidemic types and their regional characteristics of Mycobacterium tuberculosis in the two provinces and regions adjacent to the northwest of China were analyzed. Results The strains of Xinjiang were divided into 47 genotypes, of which 28 genotypes were new genotypes. Among them, Beijing families accounted for 68.72% (123/179) in Xinjiang strains, followed by T families (6.70%, 12/179), H (3.91%, 7/179) and CAS family (2.23%, 4/179). The Gansu isolates were divided into 29 genotypes, of which 10 genotypes were new genotypes. The Beijing families in Gansu strains accounted for 86.93% (153/176), followed by T family (4.55%, 8/176), H family (1.14%, 2/176), MANU2 (1.14%, 2/176) and CAS (0.57%, 1/176). Conclusions Beijing genotype strains are the major epidemic strains in Xinjiang and Gansu Provinces, but the genotype strains in Beijing are significantly higher than those in Xinjiang.