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首先通过改进的Hummers法制备了氧化石墨烯(GO),然后通过酰胺化反应将端基为氨基的六臂聚乙二醇(PEG)连接到氧化石墨烯的表面,以改善其水溶性和生物相容性.原子力显微镜(AFM)数据表明所制备的GO-PEG的尺寸小于250 nm,稳定性试验证明GO-PEG在水和PBS缓冲液中可以很好地分散.利用制备的GO-PEG作为药物载体,通过物理共混的方法负载了疏水性抗肿瘤药物——毛萼乙素.紫外光谱法测得载药率为18.8%.选择肺癌细胞A549和乳腺癌细胞MCF-7对载药体系的细胞毒性进行了研究,结果表明即使在高达100 mg/L的浓度下培养48 h,GO-PEG载体对两种细胞仍然具有很小的毒性(相对细胞存活率>85%),而通过载体负载毛萼乙素后的疗效有所增强,对癌细胞具有更大的杀伤作用.
Firstly, graphene oxide (GO) was prepared by a modified Hummers method, then six-arm polyethylene glycol (PEG) with amino group at the end was connected to the surface of graphene oxide by amidation to improve its water solubility and biological Compatibility AFM data showed that the size of GO-PEG prepared was less than 250 nm, and the stability test showed that GO-PEG could be well dispersed in water and PBS buffer.With the prepared GO-PEG as Drug carrier, the method of physical blending loaded hydrophobic antitumor drug - Calcein B. UV detection of drug-loaded rate was 18.8% .Choose lung cancer cells A549 and breast cancer cells MCF-7 drug-loaded system The results showed that the GO-PEG vector has little toxicity (relative cell viability> 85%) to both cells even when incubated at concentrations up to 100 mg / L for 48 h, Efficacy of fullegumes after loading has been enhanced, has a greater killing effect on cancer cells.