论文部分内容阅读
为评估DNA随机扩增多态性标记在中国对虾遗传连锁图谱构建中的应用前景,利用中国对虾单对交配亲本及其子二代材料,对RAPD标记及其遗传规律进行了研究。22条RAPD随机引物扩增结果的统计分析表明,标记在中国对虾F2的遗传规律可归为不分离标记和分离标记:不分离标记,指在亲本和后代中均不分离的标记,占总位点的54.1%;分离标记占总位点的45.9%。其中,分离标记又包括符合孟德尔遗传分离的标记、偏离孟德尔遗传分离标记和异常分离标记。符合孟德尔分离的标记中,分离比例为3∶1的标记占分离标记的14.7%;总的1∶1标记占分离标记的64.7%;偏离孟德尔分离和异常分离的标记分别占分离标记的11.7%和8.9%。在这些分离的标记中,有76.5%的位点在“双假测交理论”的策略中适合构建中国对虾的遗传连锁图谱,这为以中国对虾F2为作图群体,并利用RAPD标记构建中国对虾遗传连锁图谱提供了理论支持。
In order to evaluate the potential application of random amplified polymorphic DNA (DNA) polymorphism markers in the construction of genetic linkage map of shrimp in China, the RAPD markers and their genetic rules were studied using single parent mating parents and their second and second generation parents. The statistical analysis of 22 RAPD random primers showed that the genetic law of F2 in Chinese shrimp could be classified as non-segregation markers and non-segregation markers. The non-segregation markers refer to the markers that are not separated in both parents and offspring, 54.1% of the points, and 45.9% of the total sites. Among them, the segregation markers in turn include Mendelian segregation markers, Mendelian genetic segregation markers and abnormal segregation markers. Among the markers conforming to Mendelian segregation, markers with a separation ratio of 3: 1 accounted for 14.7% of the total markers; total 1: 1 markers accounted for 64.7% of the total markers; Mendelian and abnormal markers accounted for 11.7% and 8.9%. Among the segregated markers, 76.5% of the loci are suitable for constructing the genetic linkage map of P. shrimp in the strategy of “Double Fake Test”, which is based on mapping F2 population of Chinese shrimp and using RAPD markers The establishment of Chinese shrimp genetic linkage map provides theoretical support.