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目的建立了RP-HPLC法测定人血浆中头孢妥仑的浓度。方法色谱柱Nucleodur C18分析柱(4.6mm×250mm5μm),流动相为0.03%三氟乙酸缓冲液/乙腈(81/19,V/V),流速1.0ml/min,检测波长为305nm,柱温30℃,取上清液直接进样,进样量为20μL结果头孢妥仑线性范围为0.02～5.0μg/mL。头孢妥仑的最低检测限为0.02μg/mL,日内、日间RSD均小于5%,相对回收率为97.6%～104.5%,提取回收率均大于91.3%。结论这种验证方法灵敏、简便、可重复,足以用于药代动力学研究。
Objective To establish a RP-HPLC method for the determination of cefditoren in human plasma. Methods Nucleodur C18 column (4.6mm × 250mm5μm) was used as the mobile phase. The mobile phase consisted of 0.03% trifluoroacetic acid buffer / acetonitrile (81/19, V / V) at a flow rate of 1.0ml / ℃, the supernatant was injected directly into the sample volume of 20μL Results linear range of cefditoren 0.02 ~ 5.0μg / mL. The minimum detectable limit of cefditoren was 0.02μg / mL. The RSDs were less than 5%, the relative recoveries were 97.6% -104.5%, and the recovery rates were both higher than 91.3%. Conclusion This method of verification is sensitive, simple, reproducible and adequate for pharmacokinetic studies.