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以新疆野扁桃(Prunus tenella)花粉为试材,构建其自交不亲和性相关基因PetSSK1的植物表达载体pCAMBIA1303-PetSSK1,采用根癌农杆菌介导法,将该基因导入矮牵牛,并研究了不同种类及浓度抗生素对矮牵牛再生的影响。结果表明:该试验成功获得了转化新疆野扁桃自交不亲和相关基因PetSSK1的矮牵牛植株,并筛选出矮牵牛的卡那霉素(Kan)和头孢霉素(Cef)的敏感浓度分别为25、300mg·L~(-1),并进一步通过PCR分子检测确认其为转基因阳性植株。
The plant expression vector pCAMBIA1303-PetSSK1 was constructed by pollination of Prunus tenella from Xinjiang. The gene was introduced into petunia by Agrobacterium tumefaciens-mediated method The effects of different kinds and concentrations of antibiotics on the regeneration of petunia were studied. The results showed that Petunia plants transformed with PetSSK1 gene of Petunia hybrida were successfully obtained and the sensitive concentrations of Kan and Cef of Petunia hybrida were screened. Respectively 25,300mg · L -1, and further confirmed by PCR molecular detection of transgenic plants positive.