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目的:建立高效液相色谱法同时测定枫蓼肠胃康胶囊中7种黄酮类成分的含量,并对总黄酮提取工艺进行优化。方法:采用正交试验法优化总黄酮的提取工艺。含量测定色谱条件:色谱柱:Waters RPl8(250 mm×4.6 mm,5μm);流动相A:甲醇-水(80∶20),流动相B:甲醇-0.2%甲酸(17∶83),梯度洗脱;流速:1.0 ml·min~(-1);柱温:35℃;可变检测波长(0~27 min,λ_1=260 nm;27~60 min,λ_2=360 nm)。结果:7种黄酮成分在60 min内达到基线分离,线性相关系数均≥0.990 0,平均加标回收率在95.95%~97.84%之间,RSD在0.81%~1.33%之间。总黄酮提取的最佳工艺条件为溶媒倍量1∶15,大孔吸附树脂极性为弱极性(AB-8),超声提取时间30 min,乙醇体积分数60%。结论:所建立的方法操作简单,精密度高,重复性好,对枫蓼肠胃康胶囊的质量控制和检测都有一定指导意义。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of seven flavonoids in Fengliao Changweikang Capsules, and to optimize the extraction process of total flavonoids. Methods: The orthogonal experiment was used to optimize the extraction of total flavonoids. Assay conditions: Column: Waters RP18 (250 mm × 4.6 mm, 5 μm); mobile phase A: methanol-water (80:20); mobile phase B: methanol-0.2% formic acid The flow rate was 1.0 ml · min -1. The column temperature was 35 ℃. The detection wavelength was variable (0-27 min, λ_1 = 260 nm, 27-60 min, λ_2 = 360 nm). Results: Seven flavonoids were separated within 60 min. The linear correlation coefficients were all ≥0.990 0. The average recoveries ranged from 95.95% to 97.84% with RSD ranging from 0.81% to 1.33%. The optimum extraction conditions of total flavonoids were solvent 1:50, macroporous resin with weak polarity (AB-8), ultrasonic extraction time 30 min and ethanol volume 60%. Conclusion: The established method has the advantages of simple operation, high precision and good repeatability. It has certain guiding significance for the quality control and testing of Fengweijweikang capsule.