论文部分内容阅读
对具有纤溶活性的物质研究已有多年的发展,但是血栓性疾病仍然是致人死亡或身体机能障碍的主要原因之一。从具有特殊生理功能的生物体中获取活性物质近来受到广泛关注,在蚯蚓消化腺的分泌物中纯化得到一组同工酶,表现出良好的溶栓效果,并被命名为蚯蚓纤溶酶(earthworm fibrinolytic enzyme,EFE)。本文在多层次上对蚯蚓纤溶酶作一综述,内容包括:蚯蚓纤溶酶的纯化、克隆表达,并详细介绍该酶结构与功能之间的关系以及临床应用等。
Research on fibrinolytic substances has been underway for many years, but thrombotic disorders remain one of the major causes of death or physical impairment. Recently, the active substances obtained from organisms with special physiological functions have received widespread attention. A group of isoenzymes were purified from the secretions of the digestive gland of earthworms and showed a good thrombolytic effect and were named as earthworm plasmin earthworm fibrinolytic enzyme, EFE). This article reviewed the earthworm fibrinolytic enzyme at many levels, including: the purification and cloning of earthworm fibrinolytic enzyme, and the relationship between the structure and function of the enzyme and its clinical application.