We aimed to compare the sperm quality in different cancer types and benign diseases before gonadotoxic treatment,and assess the usage rate of cryopreserved sperm for assisted reproductive treatment(ART).This retrospective study was conducted at two university clinics between January 2008 and July 2018.A total of 545 patients suffering from cancer or benign diseases were included in the study.The pretreatment sperm analyses were based on the World Health Organization(WHO)guidelines.Patients with testicular malignancy(TM)showed a significantly lower sperm count(median[interquartile range]:18.7×106[5.3×106-43.0×106]ml-1;P=0.03)as well as total sperm count(42.4×106[13.3×106-108.5×106]per ejaculate;P=0.007)compared to other malignant and benign diseases.In addition,patients with nonseminomatous TM showed the lowest sperm count(14.3 x 106[6.0×106-29.9×106]ml-1,vs seminomas:16.5×106[4.6×106-20.3×106]ml-1;P=0.001).With reference to the WHO 2010 guidelines,approximately 48.0％of the patients with TM and 23.0％with hematological malignancies(HM)had oligozoospermia.During the observation period,only 29 patients(5.3％)used their frozen sperms for 48 ART cycles,resulting in 15 clinical pregnancies and 10 live births.The sperm quality varies with the type of underlying disease,with TM and HM patients showing the lowest sperm counts.Due to the observed low usage rate of cryopreserved sperm,further patient interviews and sperm analyses should be included in the routine oncologic protocols to avoid unnecessary storage expenses.However,sperm banking is worth the effort as it provides hope for men who cannot reproduce naturally after gonadotoxic treatment.