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目的:探讨大黄甘草配伍对大黄内蒽醌类成分溶出含量的影响,旨在为临床研究大黄甘草汤的作用机理提供参考。方法:采用高效液相色谱法(HPLC)测定大黄及大黄甘草汤中总蒽醌与结合型蒽醌含量,应用Kromasil C18(250mm×4.6mm,5μm)型色谱柱,以甲醇为流动相A相,1%冰醋酸溶剂系统为流动相B相,在1.0ml/min下进行线性梯度洗脱,检测波长:254nm,柱温:35℃。结果:HPLC法测定大黄及大黄甘草汤中蒽醌类成分含量,精密度、重复性、稳定性RSD均小于3%,加样回收率97.36%~104.53%;大黄单煎及大黄甘草汤中蒽醌总量为(10.472±0.023)mg/g,结合型蒽醌总量(7.387±0.054)mg/g;大黄甘草汤中蒽醌总量为(12.583±0.034)mg/g,结合型蒽醌总量(8.292±0.036)mg/g,大黄单药及与甘草配伍后样品中蒽醌类成分含量存在差异。结论:大黄配伍甘草后药材中蒽醌类成分溶出量较单味大黄提高,大黄甘草配伍可促进大黄中蒽醌类成分溶出。
Objective: To investigate the compatibility of rhubarb and licorice on the content of anthraquinones in rhubarb, and to provide a reference for the clinical study of the mechanism of rhubarb and licorice. Methods: The contents of total anthraquinone and bound anthraquinone in rhubarb and rhubarb and licorice soup were determined by high performance liquid chromatography (HPLC). The chromatographic column was Kromasil C18 (250 mm × 4.6 mm, 5 μm) , 1% glacial acetic acid as mobile phase B, linear gradient elution at 1.0ml / min, detection wavelength: 254nm, column temperature: 35 ℃. Results: The content of anthraquinones in rhubarb and rhubarb and licorice soup were determined by HPLC. The RSD of precision, repeatability and stability were all less than 3% and the recoveries were 97.36% -104.53% (10.472 ± 0.023) mg / g and the total amount of anthraquinone combined (7.387 ± 0.054) mg / g. The total amount of anthraquinones in Dahuang Gancao Decoction was (12.583 ± 0.034) mg / g, The total amount of (8.292 ± 0.036) mg / g, the content of anthraquinones in rhubarb single drug and licorice compatibility sample differences. Conclusion: The rhubarb compatibility licorice herbs anthraquinones eluted more than single rhubarb, rhubarb and licorice compatibility can promote rhubarb anthraquinones dissolution.