本文报道了用光敏生物素标记恶性疟原虫特异克隆 p BF2 DNA片断作探针 ,以斑点杂交试验检测不同疟区疟疾病人血样和蚊体内的疟原虫。探针检测蚊媒时 ,在 2 0只蚊虫中有 1只感染蚊虫即可被检出 ,也可将单个蚊虫直接压在硝酸纤维素膜上进行检测 ;探针检测血样亦取得良好结果 ,与镜检的符合率 ,恶性疟 96 .6 % ,正常人对照 99.7% ,检测的敏感度为 90个原虫 /μl血。表明该探针在疟防后期的监测中具有较好的实用性 In this paper, we report the detection of Plasmodium falciparum p BF2 DNA fragment by photo-biotin as a probe and the dot blot hybridization assay for blood samples of malaria patients and malaria parasite in different malaria areas. When the mosquito is detected by a probe, only one mosquito in 20 mosquitoes can be detected, and a single mosquito can also be directly pressed on the nitrocellulose membrane for detection. The probe also has good results in detecting blood samples, Microscopic examination of the coincidence rate, 96.6% of falciparum malaria, normal control 99.7%, detection sensitivity of 90 protozoa / μl blood. It indicates that the probe has good practicability in the monitoring of malaria in the later stage