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目的研究过表达目标蛋白(HMOX-1、MT2A、UBB、RPS27a)对人肝癌HepG2细胞增殖和侵袭力的影响。方法用目标蛋白的过表达质粒转染HepG2细胞株,用空载体转染HepG2作为对照组。应用MTT法测定细胞体外增殖能力;Transwell实验检测各组细胞的体外侵袭力。结果 MTT法结果提示过表达目标蛋白后,HepG2细胞株增殖能力较对照组明显增强,差异有统计学意义(P<0.05);Transwell实验结果表明,过表达HMOX1、RPS27a后,HepG2细胞侵袭能力较对照组明显增加,差异有统计学意义(P<0.05);过表达MT2A、UBB后,HepG2细胞侵袭能力与对照组比较差异无统计学意义(P>0.05)。结论过表达目标蛋白可增强HepG2细胞的增殖和侵袭能力。
Objective To investigate the effects of overexpressing target proteins (HMOX-1, MT2A, UBB and RPS27a) on the proliferation and invasion of human hepatoma HepG2 cells. Methods HepG2 cells were transfected with the target protein overexpression plasmid and HepG2 transfected with empty vector as control group. MTT assay was used to determine the cell proliferation in vitro. Transwell assay was used to detect the invasiveness of cells in vitro. Results The results of MTT assay showed that the proliferation of HepG2 cells was significantly higher than that of the control cells after overexpression of the target protein (P <0.05). The results of Transwell assay showed that the invasion ability of HepG2 cells over-expressing HMOX1 and RPS27a was higher than that of HepG2 cells (P <0.05). After over-expressing MT2A and UBB, the invasive ability of HepG2 cells was not significantly different from that of the control group (P> 0.05). Conclusion Overexpression of target protein can enhance the proliferation and invasion ability of HepG2 cells.