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目的 :探讨白介素 10 (IL 10 )、血小板衍生生长因子 BB (PDGF BB)对体外活化的培养大鼠肝星状细胞(HSC)表达细胞间黏附分子 1(ICAM 1)mRNA的影响。方法 :体外培养激活的HSC (细胞系rHSC 99)随机分为 4组 :对照组 (A组 )、IL 10 2 0ng/ml干预组 (B组 )、PDGF BB 2 0ng/ml干预组 (C组 )及IL 10 2 0ng/ml +PDGF BB2 0ng/ml共干预组 (D组 )。加药后 2 4小时 ,采用半定量逆转录聚合酶链反应 (RT PCR )方法 ,检测各组ICAM 1mRNA表达情况。结果 :B组ICAM 1mRNA的表达较A组明显降低 (P <0 0 1) ;C组ICAM 1mRNA的表达较A组明显增强 (P <0 0 1) ;D组ICAM 1mRNA的表达较A组与C组均降低 (P <0 0 5 ,P <0 0 1) ,但与B组相比较其ICAM 1mRNA的表达则增强 (P <0 0 1)。结论 :PDGF BB促进肝纤维化与其引起HSC表达ICAM 1增强有关 ,IL 10通过下调HSCICAM 1表达 ,可在抑制肝纤维化中发挥作用。
Objective: To investigate the effects of interleukin 10 (IL 10) and platelet derived growth factor BB (PDGF BB) on the expression of intercellular adhesion molecule 1 (ICAM 1) mRNA in cultured rat hepatic stellate cells (HSC). Methods: The activated HSCs (cell line rHSC 99) cultured in vitro were randomly divided into four groups: control group (group A), IL 10 2ng / ml intervention group (group B), PDGF BB 2ng / ml intervention group ) And IL-10 2ng / ml + PDGF BB2 0ng / ml co-intervention group (group D). Twenty-four hours after dosing, the expression of ICAM-1 mRNA in each group was detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT PCR). Results: The expression of ICAM-1 mRNA in group B was significantly lower than that in group A (P <0.01); the expression of ICAM-1 mRNA in group C was significantly higher than that in group A (P <0.01) (P <0.01, P <0.01), but the expression of ICAM 1 mRNA in group C was significantly increased (P <0.01). CONCLUSION: PDGF BB can promote hepatic fibrosis and enhance its expression of ICAM-1 in HSC. IL-10 may play a role in inhibiting hepatic fibrosis by down-regulating HSCICAM-1 expression.