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目的探索分化早期神经干细胞的电生理特征。方法分离培养胚胎大鼠海马神经干细胞。应用膜片钳全细胞记录技术检测神经干细胞和分化早期(1～3 d)神经细胞的电压门控离子电流。结果未分化的神经干细胞不表达内向钠离子电流,而诱导分化1 d的神经细胞即表达内向性钠离子电流,但不能诱导产生动作电位。神经干细胞和分化早期的神经细胞均表达两种类型的外向钾离子电流,并表达内向T-型钙电流。结论钠离子电流的功能性表达是神经干细胞退出细胞周期开始分化的标志。分化早期是神经干细胞离子通道发育过程的重要阶段。
Objective To explore the electrophysiological characteristics of neural stem cells in early differentiation. Methods Cultured hippocampal neural stem cells from embryonic rat. Patch clamp whole-cell recording technique was used to detect voltage-gated ion currents in neural stem cells and neural cells in the early stage of differentiation (1-3 days). Results Undifferentiated neural stem cells did not express inward sodium current, while the neurons that induced differentiation for 1 d expressed inward sodium currents but failed to induce action potentials. Both neural stem cells and early neural cells express two types of outward potassium currents and express inward T-type calcium currents. Conclusion The functional expression of sodium currents is a sign that neural stem cells exit the cell cycle and begin to differentiate. Early differentiation is an important phase of neural stem cell ion channel development.