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将马来丝虫微丝蚴注入实验鼠静脉,能产生持续的微丝蚴血症,从而提供了一种可用以评价杀微丝蚴药物的模型。本文报导海群生对注入鼠体内马来丝虫微丝蚴的作用。从感染的蒙古沙鼠获得微丝蚴。将动物麻醉,取20毫升 Dulbecco 磷酸缓冲液,经18号标准塑料导管注入腹腔,按摩腹腔后,回收含微丝蚴液。洗涤3次,浓缩至1毫升 pH7. 2磷酸缓冲盐液含2×10~5微丝蚴悬液。体重18～20克的雌性CF_1鼠,每只静脉内注射0. 5毫升悬液间隔一定时间,从每只鼠眶后静脉窦
Injecting Malay filarial microfilariae into the venous veins in mice resulted in sustained microfilamentosis, providing a model that could be used to evaluate the presence of microfilariae drugs. This article reports the sea qunsheng on the injection of mice Mailai microfilaria role. Microfilaria was obtained from infected Mongolian gerbils. The animals were anesthetized, and 20 ml of Dulbecco’s phosphate buffer was injected into the abdominal cavity through a standard plastic catheter. The abdominal cavity was massaged to recover microfilamentous fluid. Washed three times, concentrated to 1 ml of pH 7. 2 phosphate buffer containing 2 × 10 ~ 5 microfilariae suspension. Body weight 18 ~ 20 grams of female CF_1 mice, each intravenous injection of 0.5 ml suspension interval for a certain period of time, from each rat retro-orbital sinus