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目的比较曼氏血吸虫吡喹酮敏感株、抗性株雌性与雄性尾蚴对吡喹酮敏感性的差异,为探索血吸虫对吡喹酮抗性产生机制提供线索。方法分别用曼氏血吸虫吡喹酮敏感株、抗性株感染鼠粪便中的虫卵孵化毛蚴,以单只毛蚴感染单只光滑双脐螺,建立单性别血吸虫尾蚴系;以W1特异性序列为引物,采用直接PCR法鉴别出单性别系尾蚴的性别,分别将敏感株与抗性株的雌、雄尾蚴暴露于不同浓度的吡喹酮溶液中,经一定时间后观察并计算尾蚴的断尾率。结果曼氏血吸虫尾蚴暴露于10-4、10-5、6×10-7mol/L和4×10-7mol/L吡喹酮溶液中100min,吡喹酮敏感株雄性尾蚴的断尾率分别为66.7%、75.8%、43.5%和21.7%,雌性的断尾率分别为29.3%、27.9%、12.1%和7.6%,雄性尾蚴的断尾率显著高于雌性尾蚴(P均<0.05);而抗性株雄性尾蚴的断尾率分别为43.3%、39.4%、25.4%和6.9%,雌性尾蚴的断尾率分别为47.0%、38.9%、26.3%和6.3%,两者间差异无统计学意义(P均>0.05)。曼氏血吸虫尾蚴分别暴露于10-4、10-5、6×10-7mol/L和4×10-7mol/L吡喹酮溶液中80min,敏感株雄性尾蚴的断尾率分别为54.4%、68.6%、42.1%和16.1%,抗性株雄性尾蚴的断尾率分别为30.2%、34.4%、20.1%和2.8%,敏感株显著高于抗性株(P均<0.05)。结论曼氏血吸虫雌雄尾蚴对吡喹酮的敏感性存在差异,雄性尾蚴敏感性高于雌性。曼氏血吸虫吡喹酮抗性的产生与雄虫对吡喹酮的敏感性降低有关。
Objective To compare the sensitivity of female and male cercariae to praziquantel of schistosoma mansoni susceptible and resistant strains, and to provide clues for exploring the mechanism of schistosoma resistance to praziquantel. Methods The schistosoma mansoni praziquantel-sensitive strains and resistant strains were used to infect the eggs in mouse feces to hatch the miracidia. The single Schistosoma japonicum cercariae was infected with a single miracidium. The specific sequence of W1 was The primers were used to identify the sex of cercar larvae by direct PCR. The female and male cercariae of the susceptible and resistant strains were exposed to different concentrations of praziquantel. After a certain period of time, the cercaria larvae were counted and counted rate. Results Schistosoma mansoni cercariae exposed to 10-4, 10-5, 6 × 10-7mol / L and 4 × 10-7mol / L praziquantel solution for 100min, praziquantel sensitive strain male cercaria larvae were 66.7%, 75.8%, 43.5% and 21.7%, respectively. The tail loss rates of female cercariae were 29.3%, 27.9%, 12.1% and 7.6% respectively, while that of male cercariae was significantly higher than that of female cercaria (P <0.05) The tailed rate of male cercariae of resistant strains were 43.3%, 39.4%, 25.4% and 6.9%, respectively, while that of female cercariae was 47.0%, 38.9%, 26.3% and 6.3%, respectively. There was no significant difference between the two groups Significance (P> 0.05). Schistosoma mansoni cercariae were exposed to 10-4, 10-5, 6 × 10-7mol / L and 4 × 10-7mol / L praziquantel solution for 80min, respectively, and the cercariae of the susceptible strain had a rate of 54.4% 68.6%, 42.1% and 16.1%, respectively, and that of male cercariae of resistant strains were 30.2%, 34.4%, 20.1% and 2.8%, respectively, and the susceptible strains were significantly higher than those of resistant strains (all P <0.05). Conclusions The sensitivity of male and female Schistosoma mansoni to praziquantel is different, and male cercariae are more sensitive than female. The production of praziquantel in Schistosoma mansoni was related to the decreased sensitivity of male to praziquantel.