论文部分内容阅读
目的探讨孤独症模型动物认知能力的变化与胃肠功能的发育状况。方法采用妊娠12.5d孕鼠尾静脉注射丙戊酸钠(注射用水稀释为250g/L,600mg/kg),所产11只仔鼠为模型组幼鼠;对照组孕鼠注射同等剂量的生理盐水,所产14只仔鼠为对照组幼鼠。观察两组小鼠在生长发育过程中的变化,并记录体质量、游泳、胃肠感觉功能、胃肠运动功能、避暗反应、旷场实验的相关数据,分析其认知发育的变化及胃肠功能的发育状况。结果与对照组相比,模型组小鼠出现生长发育迟缓、游泳协调能力差、胃肠痛阈降低,胃肠运动功能亢进;认知发育方面,模型组小鼠旷场试验运动距离较短,避暗反应具有更长的潜伏期,且错误次数更少,差异有统计学意义(P<0.05)。结论孤独症模型幼鼠生长发育过程更加迟缓,胃肠感觉运动出现障碍。另外,对未知事物的探索较少,对危险刺激因素具有更强的记忆能力。
Objective To investigate the changes of cognition and the development of gastrointestinal function in autism model animals. Methods The pregnant mice were injected with sodium valproate (diluted to 250g / L, 600mg / kg) by the tail vein of pregnant rats for 12.5 days. 11 offspring of the pregnant rats were used as the model group. The pregnant rats in the control group were injected with the same dose of normal saline , The 14 pups produced for the control group pups. The changes of growth and development of the two groups of mice were observed, and the data of body weight, swimming, gastrointestinal sensory function, gastrointestinal motility, darkening response and open-field test were recorded, and their changes in cognitive development and gastric Intestinal developmental status. Results Compared with the control group, the mice in model group showed slow growth and development, poor swimming coordination ability, decreased gastrointestinal pain threshold, and hyperactivity of gastrointestinal motility. In cognitive development, the movement of model mice in open field was short, Avoidance of dark reaction has a longer incubation period, and fewer errors, the difference was statistically significant (P <0.05). Conclusion The growth and development of juvenile model of autism is more delayed and the sensory movement of the gastrointestinal tract is disturbed. In addition, there is less exploration of unknown things and more memory of dangerous stimuli.