论文部分内容阅读
小鼠４０％体表面积经６０℃，１２ｓ烧伤后胸腺细胞出现明显的病理性调亡，表现为：与对照组比，ＤＮＡ断裂（ＤＮＡＦｒａｇｍｅｎｔａｔｉｏｎ）百分率显著增高；琼脂糖凝胶电泳出现明显的细胞凋亡特有的“梯状”条带。动态观察发现烧伤后３ｈＤＮＡ断裂百分率明显升高，１２ｈ胸腺活细胞总数明显降低，而胸腺重量和细胞总数降低则于烧伤后１８ｈ才出现。结果提示：①烧伤可诱导胸腺细胞发生严重的病理性凋亡；②烧伤后胸腺细胞病理性凋亡导致的胸腺萎缩可能与烧伤后免疫力降低有关。
Mice 40% body surface area after 60 ℃, 12s burned thymus obvious pathological apoptosis, compared with the control group, DNA fragmentation (DNAFragmentation) percentage was significantly increased; agarose gel electrophoresis significant cell apoptosis Death unique “ladder-shaped” band. Dynamic observation showed that the percentage of DNA cleavage at 3h after burn injury was significantly increased, the total number of live thymus cells decreased significantly at 12h, and the decrease of thymus weight and total number of cells occurred only 18h after burn. The results suggest that: ① Burns can induce severe pathological apoptosis of thymocytes; ② Thymus atrophy caused by pathological apoptosis of thymocytes after burn may be related to the decreased immunity after burn.