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目的探讨中国人群HIV-1B亚型Nef蛋白特异性细胞毒性T细胞(CTL)反应特征及其与病毒载量以及CD4细胞数量间的关系。方法选取33例HIV-1B亚型感染者。用合成的HIV-1B亚型Nef全基因序列肽库作为抗原,ELISPOT方法检测HIV-1B亚型Nef蛋白特异性CTL反应,同时测定病毒载量及CD4细胞数量。结果70％的感染者对Nef产生特异性CTL反应,单一肽段能够被识别的频率不超过40％,应答强度为(1102±2136)SFC(斑点形成细胞数)／106 PBMC。HIV-1B亚型Nef特异性CTL应答的强度和频率之间没有显著的相关性。HIV-1 Neff特异性CTL反应强度与病毒载量间存在显著负相关,与CD4细胞数量间存在显著正相关。结论初步确定了Nef蛋白CTL应答的优势区域。这些区域主要集中在一些高度保守的氨基酸序列。提示HIV-1B亚型Nef特异性CTL应答在疾病进展中对机体具有保护性作用。
Objective To investigate the characteristics of HIV-1B subtype Nef protein-specific cytotoxic T lymphocyte (CTL) response in Chinese population and its relationship with viral load and the number of CD4 cells. Methods 33 HIV-1B subtypes were selected. The HIV-1 subtype Nef full-length peptide library was used as antigen, ELISPOT method was used to detect Nef protein-specific CTL response of HIV-1B subtype, and the viral load and CD4 cell count were also determined. RESULTS: Seventy percent of infected individuals had a specific CTL response to Nef. The frequency of single peptide recognition was less than 40% and the response intensity was (1102 ± 2136) SFC (number of plaque-forming cells) / 106 PBMC. There was no significant correlation between the intensity and frequency of Nef-specific CTL responses in the HIV-1B subtype. There was a significant negative correlation between the intensity of HIV-1 Neff-specific CTL response and viral load, and a significant positive correlation with the number of CD4 cells. Conclusion The predominant region of Nef protein CTL response was initially identified. These regions are mainly concentrated in some highly conserved amino acid sequences. Suggesting that HIV-1B subtype Nef-specific CTL responses have a protective effect on the body during disease progression.