论文部分内容阅读
目的建立同时测定紫金龙药材4种异喹啉类生物碱含量的RP-HPLC方法。方法采用Hypersil BDS C18色谱柱(4.6mm×250mm,5μm),流动相为梯度洗脱,A相:0.2%磷酸水溶液(三乙胺调pH为7.0),B相:甲醇;流速:1mL·min-1;检测波长:265nm;柱温:30℃。结果4种生物碱可达到基线分离。青藤碱、原阿片碱、紫堇定和异紫堇定的线性范围分别为0.0073～0.0657μg,0.1722～4.304μg,0.486～4.86μg和0.4870～9.7392μg,相关系数r均大于0.9990。平均加样回收率分别为97.84%(RSD=0.89%)、98.98%(RSD=0.58%)、97.14%(RSD=1.13%)和97.65%(RSD=0.74%)。结论本法精确、简便、重现性好,可用于对紫金龙药材及其制剂的质量控制。
OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of four isoquinoline alkaloids in Zijinlongdu. Methods A Hypersil BDS C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase was a gradient elution. Phase A: 0.2% phosphoric acid (adjusted to pH 7.0 with triethylamine), B phase -1; detection wavelength: 265nm; column temperature: 30 ℃. Results Four kinds of alkaloids can achieve baseline separation. The linear range of sinomenine, protopine, purpurin and isoquinuxine was 0.0073 ~ 0.0657μg, 0.1722 ~ 4.304μg, 0.486 ~ 4.86μg and 0.4870 ~ 9.7392μg respectively, the correlation coefficients r were all greater than 0.9990. The average recoveries were 97.84% (RSD = 0.89%), 98.98% (RSD = 0.58%), 97.14% (RSD = 1.13%) and 97.65% (RSD = 0.74%), respectively. Conclusion This method is accurate, simple and reproducible. It can be used for quality control of Zijinlongdu and its preparations.