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【目的】了解不同品种酿酒葡萄根围、叶围细菌群落结构。【方法】以不同品种酿酒葡萄根围、叶围样品为分析材料,采用末端限制性片段长度多态性(T-RFLP)技术研究根围、叶围细菌群落结构,并对细菌群落多样性进行分析。【结果】不同品种葡萄根围微生物优势菌群为变形菌门,不同品种葡萄叶围微生物优势菌群为放线菌目。根围Simpson指数大小顺序为白福尔>琼瑶浆>白诗南>赛美蓉>白玉霓>米勒>长相思,Shannon指数与Simpson指数基本保持一致。叶围Simpson指数最大的为白诗南,最小的为米勒,与Shannon指数基本吻合。【结论】葡萄细菌群落结构综合多样性指数和品种以及定殖部位密切相关。
【Objective】 The objective of this study was to understand the bacterial community structure of the roots and leaves of different varieties of wine grape. 【Method】 The bacterial community structures in the rhizosphere and the leaf were studied by T-RFLP using the rhizosphere and leaf sieve samples from different varieties of wine-growing plants as materials. The diversity of bacterial communities analysis. 【Result】 The results showed that the dominant microflora in the root system of the different varieties was the proteobacteria, and the dominant microflora in the grape leaves were actinomycetes. The order of Simpson index of Rhizosphere was Baifuoluo> Gemstones> Baishishan> Semillon> Baiyuzi> Miller> Sauvignon Blanc, and the Shannon index was basically the same as that of Simpson index. Leaf Simpson index is the largest of the South White, and the smallest Miller, and Shannon index basically consistent. 【Conclusion】 The comprehensive diversity index of grape bacterial community structure is closely related to the variety and the colonization site.