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目的在6-羟基多巴胺毁损纹状体鼠模型上,探讨阿扑吗啡的神经保护和神经营养作用。方法用6-羟基多巴胺毁损大鼠单侧纹状体,在毁损前15 m in注射阿扑吗啡(10 mg/kg,s.c.),连续注射11 d。毁损2周后,分别进行行为学(苯丙胺引起的旋转数目)、组织学(黑质和腹侧被盖区的酪氨酸羟化酶阳性细胞计数)的观察。结果阿扑吗啡降低苯丙胺引起的向损伤侧旋转的次数,显著降低黑质神经元的损伤(从50%降至30%)。且多巴胺细胞形状可恢复到类似正常组;阿扑吗啡对正常鼠黑质细胞数无影响,但可使腹侧被盖区细胞数显著增高37%。结论阿扑吗啡对6-羟基多巴胺毁损纹状体模型鼠的黑质和腹侧被盖区神经细胞具有显著的保护作用,且改善运动功能。同时,对正常鼠的腹侧被盖区也显示神经营养作用。
Objective To explore the neuroprotective and neurotrophic effects of apomorphine on 6-hydroxydopamine-lesioned rat striatum. Methods 6-OHDA was used to injurize unilateral striatum in rats. Apomorphine (10 mg / kg, s.c.) was injected 15 m injections and then injected continuously for 11 days. Behavioral (amphetamine-induced rotation number), histology (tyrosine hydroxylase positive cell counts of substantia nigra and ventral tegmental area) were observed separately after 2 weeks of rupture. Results Apomorphine decreased the number of amphetamine-induced injuries to the lesion side and significantly reduced the damage to substantia nigra neurons (from 50% to 30%). And dopamine cell shape can be restored to a similar normal group; apomorphine has no effect on the number of normal rat substantia nigra cells, but can make the ventral tegmental area cell number increased significantly 37%. Conclusion Apomorphine has a significant protective effect on neurons in the substantia nigra and ventral tegmental area of 6-hydroxydopamine-lesioned striatum and improves motor function. At the same time, the ventral tegmental area of normal mice also showed neurotrophic effects.