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目的 :评价羧甲基壳聚糖锌(carboxymethyl chitosan zinc,CMCSZ)及羧甲基壳聚糖锌-活性肽复合材料(carboxymethyl chitosan zinc-active peptide complex material,CMCSZP)对口腔常见致龋菌的抑菌活性。方法 :采用琼脂稀释法测定CMCSZ和CMCSZP对变形链球菌、乳酸杆菌、血链球菌、黏性放线菌的最小抑菌浓度,采用K-B纸片扩散法测定活性肽、CMCSZ和CMCSZP的抑菌环直径,比较CMCSZ和CMCSZP抑菌活性的差别,并观察p H值、温度、光照对抑菌药物稳定性的影响。采用SPSS13.0软件包对数据进行统计学分析。结果:活性肽、CMCSZ和CMCSZP对所选口腔常见致龋菌均具有抑菌活性,CMCSZ/CMCSZP对4种细菌的最小抑菌浓度分别为625、1250、1250、2500 mg/L,且同等浓度下,CMCSZP的抑菌环直径显著大于CMCSZ(P<0.05)。酸性条件有利于增强CMCSZ的抑菌活性,但对CMCSZP无显著作用(P<0.05)。CMCSZ与CMCSZP具有较好的光稳定性(P<0.05),但抑菌活性随水浴温度的升高逐渐减弱,达到85℃时其抑菌活性消失(P<0.05)。结论:CMCSZ和CMCSZP对4种致龋菌均具有抑菌活性,且有较好的光稳定性,但热稳定性较差。本研究为CMCSZ和CMCSZP在龋病预防的应用提供了理论基础。
OBJECTIVE: To evaluate the effect of carboxymethyl chitosan zinc (CMCSZ) and carboxymethyl chitosan zinc-active peptide complex material (CMCSZP) on cariogenic oral bacteria Bacteria activity. Methods: The minimum inhibitory concentrations of CMCSZ and CMCSZP against Streptococcus mutans, Lactobacillus, Streptococcus sanguis, and actinomycetes were determined by agar dilution method. The antibacterial rings of CMCSZ and CMCSZP were determined by KB disk diffusion method Diameter, CMCSZ and CMCSZP antibacterial activity were compared, and observed p H value, temperature, light on the stability of antibacterial drugs. SPSS13.0 software package for statistical analysis of the data. Results: The active peptides, CMCSZ and CMCSZP had antibacterial activities on the common cariogenic bacteria in the selected oral cavity. The minimum inhibitory concentrations of CMCSZ / CMCSZP against the four bacteria were 625, 1250, 1250 and 2500 mg / L respectively, The antibacterial ring diameter of CMCSZP was significantly larger than that of CMCSZ (P <0.05). Acidic conditions were beneficial to enhance the antibacterial activity of CMCSZ, but had no significant effect on CMCSZP (P <0.05). CMCSZ and CMCSZP had good light stability (P <0.05), but the antibacterial activity decreased with the increase of water bath temperature, and the antibacterial activity disappeared when the temperature reached 85 ℃ (P <0.05). CONCLUSION: CMCSZ and CMCSZP have antibacterial activities against four kinds of cariogenic bacteria, and have good photostability, but poor thermal stability. This study provides a theoretical basis for the application of CMCSZ and CMCSZP in caries prevention.