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目的:探讨中药单体姜黄素对东莨菪碱致小鼠记忆障碍的影响及其可能的机制。方法:用东莨菪碱建立昆明小鼠记忆获得性障碍模型,通过跳台和水迷宫实验观察姜黄素对小鼠学习记忆能力的影响,同时测定其脑组织中乙酰胆碱酯酶、谷胱甘肽过氧化物酶和丙二醛的活性。结果:姜黄素可以减少东莨菪碱所致的记忆获得性障碍小鼠跳台回避反应的错误次数(P<0.05),延长其逃避潜伏期(P<0.05),也可以缩短记忆获得障碍小鼠水迷宫中寻找平台的潜伏期(P<0.05),撤去平台后可以延长小鼠在原平台象限的停留时间(P<0.05)。姜黄素可以降低小鼠脑乙酰胆碱酯酶活性(P<0.01)、提高抗氧化酶谷胱甘肽过氧化物酶活性(P<0.01)、降低脂质过氧化产物丙二醛含量(P<0.01)。结论:姜黄素对记忆获得性障碍有明显的改善作用,其机制可能与影响中枢胆碱能系统、抗氧化损伤有关。
OBJECTIVE: To investigate the effect of curcumin, a traditional Chinese medicine, on memory impairment in scopolamine-induced mice and its possible mechanism. METHODS: A memory-acquired disorder model of Kunming mice was established with scopolamine, and the effect of curcumin on learning and memory abilities of mice was observed through a step-up and water maze test. At the same time, acetylcholinesterase and glutathione peroxidase in brain tissue were measured. And malondialdehyde activity. RESULTS: Curcumin could reduce the number of mistakes in the evasion response of the mitochondria-induced memory-acquired mice (P<0.05), prolonged their escape latency (P<0.05), and could also reduce the number of memory-obtained mice in the water maze. The latency period of the platform (P<0.05), the withdrawal time of the mouse can be extended in the quadrant of the original platform (P<0.05). Curcumin can reduce the activity of acetylcholinesterase in mice brain (P<0.01), increase the activity of antioxidant enzyme glutathione peroxidase (P<0.01), and reduce the content of lipid peroxidation product malondialdehyde (P<0.01). ). CONCLUSION: Curcumin can significantly improve memory-acquired disorders, and its mechanism may be related to central cholinergic system and anti-oxidative damage.