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目的对近年诱导多能干细胞(induced pluripotent stem cells,i PS)体外诱导分化为雪旺细胞的相关研究进展进行综述。方法查阅目前i PS体外诱导分化为雪旺细胞的相关文献,对i PS体外分化为雪旺细胞的诱导方案以及对分化形成的雪旺细胞进行鉴定和功能性检测方法进行总结。结果研究表明i PS可诱导分化为雪旺细胞,但目前需要先将i PS诱导分化为神经嵴细胞或神经嵴干细胞,再将其进一步分化为雪旺细胞。S100-β和胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)为公认的雪旺细胞标记物。当神经嵴细胞或神经嵴干细胞分化前标记为p75~+、HNK1~+或nestin~+,诱导后标记为S100-β~+、GFAP~+,表明诱导产生的是雪旺细胞。结论将i PS诱导分化为雪旺细胞的研究虽然已有进展,但目前成功的诱导方案甚少,仍需对其进行深入研究。
OBJECTIVE: To summarize the recent advances in the induction and differentiation of induced pluripotent stem cells (iPS) into Schwann cells in vitro. Methods The current literatures about iPS-induced differentiation into Schwann cells in vitro were reviewed. The inducing program of iPS in vitro to Schwann cells and the identification and functional detection of Schwann cells were summarized. Results Studies have shown that iPS can induce the differentiation of Schwann cells, but at present it is necessary to induce i PS to differentiate into neural crest cells or neural crest stem cells, and further differentiate into Schwann cells. S100-β and glial fibrillary acidic protein (GFAP) are recognized Schwann cell markers. When neural crest cells or neural crest stem cells were labeled with p75 ~ +, HNK1 ~ + or nestin ~ + before differentiation, they were labeled as S100-β ~ + and GFAP ~ +, indicating that Schwann cells were induced. Conclusion Although the progress of the induction and differentiation of i PS into Schwann cells has been progressing, there are few successful induction regimens so far.