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目的建立测定壳聚糖微球中盐酸左氧氟沙星含量的高效液相色谱法。方法采用Agilent 1200型高效液相色谱仪,色谱柱为ZORBAX Eclipse XDB-C18(4.6 mm×150 mm,5μm),柱温:30℃,流速:1.0 mL.min 1,流动相:0.04 mol.L 1磷酸溶液(三乙胺调pH值为4)-乙腈(85∶15),检测波长:293 nm。结果盐酸左氧氟沙星浓度在1.021~51.15μg.mL 1内线性关系良好(r=0.999 6),日内和日间精密度RSD均<2%(n=5),平均加样回收率为99.1%,RSD为0.72%。结论本方法简便易行、灵敏度高、重复性好,结果准确可靠,适用于壳聚糖微球中盐酸左氧氟沙星含量测定。
Objective To establish a HPLC method for the determination of levofloxacin hydrochloride in chitosan microspheres. Methods The Agilent 1200 High Performance Liquid Chromatograph was used. The column was ZORBAX Eclipse XDB-C18 (4.6 mm × 150 mm, 5 μm). The column temperature was 30 ℃, the flow rate was 1.0 mL.min 1, the mobile phase was 0.04 mol·L 1 phosphoric acid solution (triethylamine adjusted to pH 4) - acetonitrile (85:15), detection wavelength: 293 nm. Results The linearity of levofloxacin hydrochloride was 1.021 ~ 51.15μg.mL 1 (r = 0.999 6). The intra-day and inter-day RSDs were less than 2% (n = 5), and the average recoveries were 99.1% 0.72%. Conclusion The method is simple, sensitive, reproducible and accurate. It is suitable for the determination of levofloxacin hydrochloride in chitosan microspheres.