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作者采用DEAE-Spherodex LS的填充床和扩张床,研究了从粗制人血浆蛋白混合物中分离α1-抗胰蛋白酶[亦称为α1-蛋白酶抑制剂,即α1-PI]时流动相速度、样品浓度、负载量、洗涤循环及相应纯化因素对回收率的影响。用加入含有人血清白蛋白(HSA)的原料(用于分离α1-PI的人血浆蛋白制剂常污染大量白蛋白)在扩张床上,测定扩张床中的流动相速度、对这一吸附剂弥散数(Dv)及弥散系数(D)的影响。用DEAE-Spheroex LS检测自人血浆制得的含多种蛋白的原料时,对于
Using a packed bed and an expanded bed of DEAE-Spherodex LS, the authors studied the mobile phase velocity of alpha1-antitrypsin [also known as alpha1-protease inhibitor, α1-PI] isolated from a crude human plasma protein mixture, Concentration, loading, washing cycle and the corresponding purification factors on the recovery rate. The mobile phase velocity in the expanded bed was measured on an expanded bed by adding a raw material containing human serum albumin (HSA) (human plasma protein preparation for isolating? 1-PI often contaminated with a large amount of albumin) (Dv) and dispersion coefficient (D). When DEAE-Spheroex LS is used to detect multi-protein containing materials prepared from human plasma,