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目的 评估乌司他丁(UTI)对幼猪胰肾联合移植术(SPK)后肺脏保护作用的效果.方法 将24只幼猪配成12对,再随机分为两组,每组12只.复制SPK模型.UTI组动物于麻醉后持续静脉泵注UTI 15 kU·kg~(-1)·h~(-1)至手术结束;对照组给予等量生理盐水.各组分别于术前(T0)、受体动静脉吻合完毕开放时(T1)、开放后1 h(T2)、开放后2 h(T3)、术毕(T4)采集外周动脉血3 ml,测定血浆超氧化物歧化酶(SOD)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)和白细胞介素-8(IL-8)水平;术后取肺组织观察病理变化.结果 与T0相比,对照组T1～4时血浆MDA浓度升高(P均0.05);UTI组T1～2及T4时血浆SOD活性均升高(P均0.05).与对照组相比,UTI组T1～2时血浆MDA浓度降低,T2时SOD活性升高(P均0.05),但UTI组T1～2及T4时血浆IL-8浓度较对照组下降(P均<0.05).光镜下观察UTI组肺组织病理变化较对照组明显减轻.结论 UTI能抑制中性粒细胞在肺内的聚集,抑制炎症因子的表达,减少氧自由基的产生,从而对损伤肺组织具有保护作用.“,”Objective To evaluate the effects of ulinastatin(UTI) on lung injury induced by simultaneous pancreas-kidney transplantation in piglet. Methods With reproduction of simultaneous pancreas-kidney transplantation model in piglets,12 couples of piglets were randomly divided into two groups (n=12). Piglets in UTI group were given a constant infusion of UTI 15 kU·kg~(-1)·h~(-1) by means of a pump during operation. Control group were treated with constant pumped infusion of 0.9% saline in equal volume. The blood samples were collected to measure the plasma concentrations of superoxide dismutase (SOD),malondialdehyde (MDA),tumor necrosis factor-α (TNF-α) and interleukin-8 (IL-8) before operation (baseline levels,T0),at the time of re-establishment of circulation with successful an astomosis of arteries and veins (T1),at 1 hour and 2 hours after re-establishment of circulation (T2 and T3),and at the end of operation (T4). The pathological changes in the lungs were examined. Results In control group,plasma MDA concentration was significantly increased from T1 till T4 as compared with T0 (all P0.05). In group UTI,SOD activity was significantly increased at T12 and T4 as compared with T0 (all P0.05). The plasma MDA concentration was significantly decreased at T1 and T2,and SOD activity was increased at T2 in group UTI than those in control group (all P<0.05). In control group,plasma TNF-α concentration was significantly increased from T2 to T4 as compared with T0 (all P0.05). The plasma IL-8 concentration was significantly decreased at T12 and T4 in group UTI compared with those in control group (both P<0.05). Conclusion UTI can inhibit neutrophil aggregation in lungs and expression of harmful inflammatory cytokines,and it reduces production of oxygen free radical,so that it can protect lung tissue from injury induced by simultaneous pancreas-kidney transplantation.