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目的观察琐琐葡萄多糖(VTP)对卡介苗(BCG)联合脂多糖(LPS)诱导小鼠免疫性肝损伤的保护作用,并探讨其作用机制。方法昆明种雄性小鼠72只,随机分为正常对照组、模型组、联苯双酯(DDB)阳性对照组以及VTP低、中、高剂量组共6组。除正常对照组外,其余各组均经尾静脉注射BCG致敏,次日起,除正常对照组和模型组给予蒸馏水外,阳性对照组灌胃给予DDB溶液200mg/kgbw,VTP低、中、高剂量组分别灌胃给予VTP溶液50、150、300mg/kgbw,连续12d。D12,除正常对照组外,其余各组均经尾静脉注射LPS,12h后采血,取肝脏、脾脏,分别测定脏器系数、生化指标、免疫指标,并观察肝脏病理组织学变化。结果VTP可显著降低免疫性肝损伤小鼠的肝脏系数和脾脏系数;并可显著升高小鼠肝组织中ALT、AST和SOD活性,降低MDA和NO含量;各组小鼠外周血中CD4+、CD8+细胞亚群比例无显著变化,但VTP可在一定程度上调节INF-γ、IL-2、IL-4和IL-10的分泌,恢复Th1/Th2细胞的平衡;病理组织学检查结果显示VTP可改善肝组织病理损伤,与生化检查结果相一致。结论VTP对小鼠免疫性肝损伤具有一定的保护作用,其作用机制可能与VTP的抗氧化功能及免疫调节功能有关。
Objective To observe the protective effect of VTP on immune hepatic injury induced by BCG combined with lipopolysaccharide (LPS) in mice and to explore its mechanism. Methods A total of 72 Kunming male mice were randomly divided into normal control group, model group, biphenyl ester (DDB) positive control group and VTP low, middle and high dose groups. Except for the normal control group, all other groups were sensitized with tail vein injection of BCG. From the next day, except for the normal control group and model group given distilled water, the positive control group was given intragastric administration of DDB solution 200mg/kgbw, VTP low, medium, The high-dose group was orally administered VTP solution 50, 150, 300mg/kgbw for 12 consecutive days. D12, except for the normal control group, the other groups were injected with LPS via the tail vein, and blood was taken 12 hours later. The liver and spleen were taken and the organ coefficient, biochemical index and immune index were measured, and the histopathological changes of the liver were observed. Results VTP can significantly reduce the liver coefficient and spleen coefficient of mice with immunological liver injury, and significantly increase the activity of ALT, AST and SOD, and decrease the content of MDA and NO in liver tissue; CD4+ in peripheral blood of mice in each group. There was no significant change in the proportion of CD8+ cell subsets, but VTP could regulate the secretion of INF-γ, IL-2, IL-4 and IL-10 to a certain extent and restore the balance of Th1/Th2 cells. Histopathological examination showed VTP. Can improve liver pathological damage, consistent with the results of biochemical tests. Conclusion VTP has a certain protective effect on immunological liver injury in mice, and its mechanism may be related to the antioxidant and immunoregulatory functions of VTP.