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以大白菜小孢子培养获得的单倍体植株为材料,观察花粉母细胞减数分裂行为,测定花粉生活力和单倍体植株杂交的结实性,并对子代植株的染色体进行了鉴定。结果表明:大白菜单倍体减数分裂不规则,中期Ⅰ单价体游离于赤道板附近或排列在赤道板上,可见1~2个局部联会的二价体或1个局部联会的三价体;后期Ⅰ单价体大多能够移向两极,包含0/10、1/9、2/8、3/7、4/6和5/5等分离方式,少数移向三极,包含1/4/5和2/2/6等分离方式;减数分裂产物包含二分孢子、三分孢子和四分孢子等;单倍体植株与二倍体植株杂交表现高度不育,平均每荚结籽仅为0.068粒;11个子代植株中10株为二倍体(2n=20),1株为单体(2n–1=19)。
The haploid plants obtained from microspore culture of Chinese cabbage were used as material to observe the meiosis behavior of pollen mother cells. The pollen viability and the seed setting of haploid hybrids were determined. The chromosomes of the offspring plants were also identified. The results showed that the haploid meiosis of Chinese cabbage was irregular, and the monovalent products of metaphase Ⅰ were dissociated near the equatorial plate or arranged on the equatorial plate, showing that the bivalents of one or two local unions or the tri The majority of the late I monovalent species can move to the bipolar, including the 0 / 10,1 / 9,2 / 8,3 / 7,4 / 6 and 5/5 and other separation methods, a few move to the three poles, including 1 / 4/5, and 2/2/6, etc. The meiosis product contained two spores, three spores and four spores and so on. The hybridization between haploid plants and diploid plants was highly sterile, Only 0.068; 10 out of 11 progeny plants were diploid (2n = 20) and 1 was monomer (2n-1 = 19).