论文部分内容阅读
应用Q SepharoseFastFlow对Vero细胞基质制备的I型口服脊髓灰质炎减毒活疫苗悬液进行纯化。病毒液经滤过澄清和超滤浓缩 ,获得 85%的病毒感染性滴度回收率 ,而经Q SepharoseF .F .纯化的病毒悬液 ,病毒感染性滴度回收率达 10 0 %。纯化后的病毒液用α 32 PdATP标记DNA探针膜杂交法测定 ,宿主Vero细胞基质DNA残余含量远低于 10 0 pg/剂量的标准 ;rct/ 4 0特征、病毒形态及病毒衣壳蛋白组份等生物学性状无显著变化。研究结果提示 ,Q SepharoseF .F .是Vero细胞制备口服脊髓灰质炎减毒疫苗的理想纯化材料。
The live attenuated form I oral poliovirus vaccine prepared from the Vero cell matrix was purified using Q SepharoseFastFlow. The virus solution was clarified by filtration and concentrated by ultrafiltration to obtain a virus recovery titer recovery of 85%. The virus titer recovered by Q Sepharose F.F. virus suspension was 100%. The purified virus solution was assayed by α 32 PdATP-labeled DNA probe membrane hybridization and the residual content of host cell Vero cell matrix DNA was much lower than the standard of 10 0 pg / dose. The rct / 40 characteristics, virus morphology and viral capsid protein No significant changes in biological characteristics such as copies. The results suggest that Q Sepharose F. F. Is an ideal purification material for the preparation of attenuated oral polio vaccine in Vero cells.