【摘 要】
：
目的 探索乔松素(PIN)对对乙酰氨基酚(APAP)诱发肝损伤小鼠模型的保护作用.方法 50只健康雄性C57BL/6J小鼠随机分成空白组、PIN(50 mg/kg)组、APAP(300 mg/kg)模型组、PIN(30
【机 构】
：
西南医科大学附属医院四川省院士(专家)工作站,四川泸州646000;西南医科大学附属医院肝胆外科,四川泸州646000;西南医科大学附属医院肝胆外科,四川泸州,646000
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目的 探索乔松素(PIN)对对乙酰氨基酚(APAP)诱发肝损伤小鼠模型的保护作用.方法 50只健康雄性C57BL/6J小鼠随机分成空白组、PIN(50 mg/kg)组、APAP(300 mg/kg)模型组、PIN(30 mg/kg)+APAP(300 mg/kg)组和PIN(50 mg/kg)+APAP (300 mg/kg)实验组,每组各10只.各组采取灌胃给药,空白对照组和模型组给予等量的生理盐水,PIN组及PIN+APAP组每日给药1次,连续给药7 d.末次给药2 h后,模型组和PIN+APAP组腹腔注射APAP 300 mg/kg 1次,空白组和PIN组腹腔注射等量生理盐水.收集血清,检测ALT、AST水平,肝组织匀浆检测丙二醛( MDA)、超氧化物歧化酶( SOD)及谷胱甘肽( GSH)生化指标,HE染色观察肝脏组织病理情况.计量资料多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验.结果 与空白组比校,APAP (300 mg/kg)模型组的ALT、AST水平显著升高(P＜0. 01),模型构建成功;PIN(30 mg/kg)+APAP(300 mg/kg)及PIN(50 mg/kg)+APAP (300 mg/kg)组的ALT、AST与APAP(300 mg/kg)模型组相比均显著降低(P值均＜0. 01).与空白组比校,APAP(300 mg/kg)模型组小鼠肝脏中MDA的水平显著升高(P＜0. 01),且SOD活性及GSH水平显著降低(P＜0. 01);与APAP(300 mg/kg)模型组比较,PIN(30 mg/kg)+APAP(300 mg/kg)及PIN(50 mg/kg)+APAP(300 mg/kg)组的小鼠肝脏中MDA的水平显著降低( P值均＜0. 05),且SOD活性及GSH水平显著升高(P值均＜0. 05).组织病理观察显示PIN可明显改善APAP对肝组织的损伤,使肝组织形态相对正常.结论PIN对APAP诱发的小鼠肝损伤具有明显的保护作用,其保护作用可能与抑制肝脏氧化应激相关.
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