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[目的]:通过探讨奥曲肽干预人肝星状细胞株LX-2后的细胞增殖和凋亡情况以及STAT3、SOCS3的表达水平,进一步研究奥曲肽对人肝纤维化进程可能的调控机制。[方法]体外培养LX-2,使用不同浓度的奥曲肽(10-3、10-4、10-5、10-6 mmol/L)作用于LX-2 24h后,采用MTT法和TUNEL荧光法分别检测LX-2的增殖和凋亡情况,采用免疫细胞化学法检测STAT3、SOCS3蛋白水平的表达,采用RT-PCR法检测STAT3和SOCS3mRNA水平的表达。[结果]奥曲肽可以降低LX-2的增殖速度,并促进其凋亡;同时,奥曲肽能够下调STAT3、SOCS3在蛋白及mRNA水平的表达。[结论]奥曲肽可以通过抑制LX-2增殖、促进其凋亡从而延缓肝纤维化进展,并且该作用可能是通过抑制STAT3、SOCS3的表达实现的。
[Objective] The purpose of this study was to investigate the possible regulation of octreotide on the progression of human hepatic fibrosis by investigating the effects of octreotide on the proliferation and apoptosis of human hepatic stellate cell line LX-2 and the expression of STAT3 and SOCS3. [Method] LX-2 was cultured in vitro and treated with different concentrations of octreotide (10-3, 10-4, 10-5 and 10-6 mmol / L) for LX-2 for 24 h. MTT assay and TUNEL fluorescence assay The proliferation and apoptosis of LX-2 were detected. The expressions of STAT3 and SOCS3 were detected by immunocytochemistry. The expressions of STAT3 and SOCS3 mRNA were detected by RT-PCR. [Results] Octreotide could reduce the proliferation rate of LX-2 and promote its apoptosis. At the same time, octreotide down-regulated the expression of STAT3 and SOCS3 protein and mRNA. [Conclusion] Octreotide can delay the progression of hepatic fibrosis by inhibiting the proliferation of LX-2 and promoting its apoptosis, and this effect may be through inhibiting the expression of STAT3 and SOCS3.