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用含有二甲基氟磺酸及胰酶抑制剂的提取液从乳腺癌组织中提取细胞膜成份及细胞可溶性蛋白,后者再经ConA-葡聚糖柱层析获得乳腺癌细胞的全糖蛋白,经SDS-聚丙烯酰胺密度
Cell membrane fractions and cell-soluble proteins were extracted from breast cancer tissues using an extract containing dimethylfluorosulfonic acid and trypsin inhibitor, and the latter was extracted by ConA-dextran column chromatography to obtain the full glycoprotein of breast cancer cells, After SDS-polyacrylamide density