目的探讨ＰＣＲ在女性生殖道ＨＰＶ感染诊断中的价值。方法对临床诊断为尖锐湿疣及假性湿疣的７０例患者，取活体标本及宫颈分泌物做病理学及聚合酶链反应检查。结果ＰＣＲ检查活体标本尖锐湿疣阳性率为１００％；假性湿疣为５１％；宫颈分泌物ＰＣＲ，尖锐湿疣阳性率为９５６％；假性湿疣阳性率为５４％，与活体标本ＰＣＲ检查无显著性差异且与组织病理学检查有显著性差异。结论ＰＣＲ技术诊断人乳头瘤病毒感染诊断率高，认为假性湿疣与ＨＰＶ感染有关。 Objective To investigate the value of PCR in the diagnosis of female genital tract HPV infection. Methods Seventy patients with condylomata acuminata and condylomata acuminatum were examined by pathology and polymerase chain reaction. Results The positive rate of genital warts was 100% in PCR and 51% in genital warts. The positive rate of cervical secretions PCR and genital warts was 95.6%. The positive rate of genital warts was 54% Significant differences and histopathological examination were significantly different. Conclusion PCR diagnosis of human papillomavirus infection has a high diagnostic rate, which is considered to be related to HPV infection.