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目的探讨长期铅暴露大鼠铁过载对神经元的损伤。方法 SD大鼠母代与子代分别设对照、低、高剂量铅暴露组(母代:800、1 500 mg/L,子代:300、900 mg/L乙酸铅饮水方式染毒)连续70周;电感耦合等离子体发射光谱仪(ICP-AES)测血液和海马元素铅及铁,同部位海马石蜡切片分别行铁染色及硫堇染色并进行图片融合。结果染铅后,大鼠血和海马铅及海马铁含量均增加(P<0.01);低、高剂量铅暴露组子代大鼠海马CA3区铁沉积IOD值分别为(5 930.71±2 517.68)和(1 1382.43±2 551.14),均高于对照组的(3 786.78±1 256.37),差异有统计学意义(P<0.01);低、高剂量铅暴露组子代大鼠海马CA1区的神经元数量分别为(70.71±11.80)、(70.37±17.53)个/视野,均低于对照组的(83.71±12.60)个/视野,差异有统计学意义(P<0.01);图像融合显示,染铅大鼠神经元内铁沉积增加而神经元数量及层数减少,损伤加重。结论基于铁染色和硫堇染色图像融合可直观地观察铅暴露大鼠铁过载部位神经元损伤。
Objective To investigate the neuronal damage induced by iron overload in long-term lead exposure rats. Methods Sprague-Dawley rats exposed to low and high doses of lead (800,1 500 mg / L, 300,900 mg / L lead acetate) The contents of lead and iron in blood and hippocampus and the paraffin sections of hippocampus in the same area were measured by inductively coupled plasma atomic emission spectrometry (ICP-AES). Results After lead exposure, the contents of lead and hippocampal iron in rat blood and hippocampus increased (P <0.01). The IOD values of hippocampal CA3 iron deposition in offspring of low and high dose lead exposure groups were (5 930.71 ± 2 517.68) And (1382.43 ± 2 551.14), respectively, which were significantly higher than those in the control group (3 786.78 ± 1 256.37) (P <0.01). The nerves in hippocampal CA1 region of offspring of rats exposed to low and high doses of lead (70.71 ± 11.80) and (70.37 ± 17.53) / visual fields, respectively, which were lower than those in the control group (83.71 ± 12.60) per field of vision, the difference was statistically significant (P <0.01) In the lead rats, the iron deposition in neurons increased while the number and number of neurons decreased. Conclusion The neuronal injury caused by iron overload in lead exposed rats can be visually observed based on the image fusion of iron staining and thionine staining.