论文部分内容阅读
目的:探讨青春双歧杆菌细胞壁对小鼠腹腔巨噬细胞功能的影响及其对人膀胱癌细胞的体内抑制作用。方法:将青春双歧杆菌细胞壁腹腔注射于KM小鼠,以ELISA法测定腹腔巨噬细胞产生的IL-1、IL-12及TNF-α的含量;建立膀胱癌小鼠模型,腹腔注射双歧杆菌细胞壁,2周后,剥离瘤体,计算抑瘤率。结果:青春双歧杆菌细胞壁注射组腹腔巨噬细胞产生的IL-1、IL-12及TNF-α的含量分别高于对照组,两者比较具有显著差异(p<0.01);细胞壁注射组可以使小鼠局部肿瘤体积减小、肿瘤重量减轻,与对照组之间比较有显著性差异(p<0.01)。结论:青春双歧杆菌细胞壁成分能激活小鼠腹腔巨噬细胞,并且在体内能抑制人膀胱癌细胞的生长。
Objective: To investigate the effect of Bifidobacterium adolescentis cell wall on the function of murine peritoneal macrophages and its inhibitory effect on human bladder cancer cells in vivo. Methods: The cell wall of Bifidobacterium adolescentis was injected intraperitoneally into KM mice. The levels of IL-1, IL-12 and TNF-α produced by peritoneal macrophages were measured by ELISA. The mouse model of bladder cancer was established. Bacillus cell wall, 2 weeks later, peel the tumor, calculate the inhibition rate. Results: The levels of IL-1, IL-12 and TNF-α produced by peritoneal macrophages in Bifidobacterium adolescentis were significantly higher than those in control group (p <0.01). Cell wall injection group Mice reduced the size of local tumor, tumor weight, compared with the control group were significantly different (p <0.01). Conclusion: Bifidobacterium adolescentis cell wall components can activate mouse peritoneal macrophages, and can inhibit the growth of human bladder cancer cells in vivo.