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目的 观察胃良、恶性上皮组织表达GrB的情况并探讨其对局部免疫反应的影响。方法 采用免疫组织化学染色S P法检测慢性胃炎 (8例 )及胃癌组织 (35例 )中GrB的表达及组织中浸润的GrB+ CTL、NK细胞密度。结果 (1)胃良、恶性上皮组织均有GrB表达 ,阳性率分别是 6 2 5 % (5 8)和 4 0 % (14 35 ) ,差异无显著意义 (χ2 =0 5 8,P >0 0 5 ) ;(2 )胃癌组GrB+ CTL、NK细胞密度高于良性病变组 ,但差异亦无显著意义 (t=1 6 3,P >0 0 5 ) ;(3)癌组织GrB表达及其浸润的GrB+ CTL、NK细胞密度在各临床病理组间差异均无统计学意义 ,但前者在年龄组的差异接近检验水准 (P =0 0 6 ) ;(4)GrB+ 胃癌与GrB- 比较 ,其间质GrB+ CTL、NK细胞密度显著下降 (t=1 89,P <0 0 5 ) ,两者呈显著负相关 (γs=- 0 5 5 ,P <0 0 0 1)。结论 GrB表达可能是胃上皮细胞恶性转化过程伴随的改变之一 ,为转化细胞提供免疫保护 ,并可能是肿瘤逃逸免疫应答的新的分子机制。
Objective To observe the expression of GrB in benign and malignant gastric epithelium and to investigate its effect on the local immune response. Methods The expression of GrB, CTL and NK cell density in chronic gastritis (8 cases) and gastric carcinoma (35 cases) were detected by immunohistochemical staining (SP method). Results (1) The expression of GrB in gastric benign and malignant epithelial tissues was 62.5% (5 8) and 40% (14 35%) respectively, with no significant difference (χ2 = 0 58, P 0 (2) The density of GrB + CTL and NK cells in gastric cancer group was higher than that in benign lesions group, but the difference was not significant (t = 16.3, P> 0.05). (3) The expression of GrB The infiltration of GrB + CTL, NK cell density in each clinicopathological group had no statistical significance, but the difference of the former in the age group was close to the test level (P = 0 06); (4) Compared with GrB- Interstitial GrB + CTL, NK cell density decreased significantly (t = 1 89, P <0 05), the two showed a significant negative correlation (γs = - 0 55, P 0 01). Conclusion GrB expression may be one of the accompanying changes in the malignant transformation of gastric epithelial cells, providing immunoprotection for transformed cells and may be a new molecular mechanism of tumor escape immune response.