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目的了解我国部分林区鼠中莱姆病螺旋体感染及其基因分型情况。方法应用巢式PCR扩增鼠中莱姆病螺旋体5S-23S rRNA间隔区片段,对阳性产物进行限制性片段长度多态性(RFLP)分析。结果共检测鼠8属19种455只,感染莱姆病螺旋体的有4属8种26只,感染率为5.71%。内蒙古、黑龙江、浙江、贵州林区鼠的感染率分别为3.45%、4.84%、8.00%、7.14%;其中东北林区(包括内蒙古和黑龙江)棕背鼠平的感染率为9.09%;浙江林区社鼠的感染率为9.26%;未发现新疆林区鼠感染莱姆病螺旋体。RFLP分析结果显示内蒙古、黑龙江林区鼠中莱姆病螺旋体均为B.garinii基因型,而浙江、贵州林区鼠感染的莱姆病螺旋体包括B.garinii和B.valaisiana两种基因型。结论东北林区及浙江林区以B.garinii基因型为主,棕背鼠平、社鼠可能分别是两地林区莱姆病螺旋体的主要储存宿主。
Objective To understand the spirochete infection and genotyping of Lyme disease in some forest areas in China. Methods A 5S-23S rRNA spacer fragment of Borrelia burgdorferi was amplified by nested PCR. The positive products were analyzed by restriction fragment length polymorphism (RFLP). Results A total of 455 mice belonging to 8 genera and 19 species were detected. There were 4 genera, 8 species and 26 species of Borrelia burgdorferi, the infection rate was 5.71%. Infection rates of rats in Inner Mongolia, Heilongjiang, Zhejiang and Guizhou were 3.45%, 4.84%, 8.00% and 7.14%, respectively. The infection rate of brownback mice in the forest regions of Northeast China (including Inner Mongolia and Heilongjiang) was 9.09% District community rat infection rate was 9.26%; no discovery in Xinjiang forest mouse infection Lyme disease spirochete. The result of RFLP analysis showed that the Borrelia burgdorferi isolates were B.garinii in Inner Mongolia and Heilongjiang forests, while the Borrelia burgdorferi isolates in Zhejiang and Guizhou forests included B.garinii and B.valaisiana genotypes. Conclusion The B.garinii genotype is the dominant species in the forest regions of Northeast China and Zhejiang Province. The brown and white rats may be the main storage hosts for Borrelia burgdorferi in the forestlands of the two regions.