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以大豆“黑农37”胚尖为外植体,利用农杆菌介导法将抗除草剂基因EPSPS转入大豆,并对转化各培养阶段6-苄氨基腺嘌呤(6-BA)浓度进行了优化。结果表明:在预培养和共培养培养基中加入较高浓度6-BA有利于抗性芽的诱导;1mg/L6-BA和0.2mg/L吲哚丁酸(IBA)配合使用有利于抗性芽的伸长且提高了抗性芽的再生率;将抗性芽添加在1mg/L IBA的培养基中培养7d后转入不加任何激素的培养基中生根快且移栽后成活率高。PCR结果初步证明将EPSPS基因转入到大豆中。
The embryo tips of soybean “Heinong 37” were used as explants, and the herbicide resistance gene EPSPS was transferred into soybean by Agrobacterium tumefaciens-mediated method. The transformation of 6-benzyladenine (6-BA) Optimized. The results showed that the higher concentration of 6-BA in the pre-culture and co-culture medium was conducive to the induction of resistant shoots; the combination of 1mg / L 6-BA and 0.2mg / L indole butyric acid (IBA) The elongation of shoots increased the regeneration rate of resistant shoots. The resistant shoots were added to 1mg / L IBA medium for 7 days and then transferred to medium without any hormones for rapid rooting and high survival rate after transplanting . PCR results initially demonstrated that the EPSPS gene was transferred into soybean.