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目的:分析比较产地生态因子对金银花活性成分的影响,为金银花适宜种植区域确定提供依据。方法:于2014年5—7月份在全国金银花种植区海拔在4~2 100 m采得11个海拔高度上的金银花样本,经HPLC分析得到10种活性成分含量,同时获取各样地相应的气候因子,用SPSS 17.0统计软件对所得数据进行相关性分析和逐步线性回归分析。结果:不同产地金银花活性成分含量差异显著;海拔高度是影响绿原酸与木犀草苷的主导生态因子,随着金银花种植区海拔的升高,绿原酸和木犀草苷含量呈增加趋势;咖啡酸含量与7月份最高温呈显著正相关,与日照时数呈显著正相关;异绿原酸A含量与降雨量呈显著正相关;槲皮素含量与1月份最低温度呈显著正相关。结论:根据本实验研究结果推测,金银花种植区随着海拔升高,花期温度降低,有利于绿原酸和木犀草苷合成。
OBJECTIVE: To analyze and compare the effects of different ecological factors on the active constituents of honeysuckle, and provide the basis for determining the suitable planting regions of honeysuckle. Methods: From July to July in 2014, Honeysuckle samples collected at eleven altitudes from 4 to 2 100 m above sea level in the honeysuckle planting area of China were collected. The contents of 10 active ingredients were obtained by HPLC analysis. At the same time, The climate factors, using SPSS 17.0 statistical software on the data correlation analysis and stepwise linear regression analysis. Results: The content of active ingredients of honeysuckle in different areas was significantly different. Altitude was the dominant ecological factor affecting chlorogenic acid and luteolin, and the content of chlorogenic acid and luteolin increased with the elevation of honeysuckle planting area. There was a significant positive correlation between acid content and the highest temperature in July and a positive correlation with sunshine hours. There was a significant positive correlation between the content of chlorogenic acid A and rainfall. The content of quercetin was positively correlated with the lowest temperature in January. Conclusion: According to the results of this experiment, it is inferred that as the altitude increases, the temperature of flowering decreases with the increase of altitude, which is favorable for the synthesis of chlorogenic acid and luteolin.