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目的为干细胞治疗研究构建2型糖尿病(T2DM)树闙模型,并对相关因素进行分析。方法采用高糖、高脂饲喂联合腹腔注射链脲佐菌素(STZ)100 mg/kg方法构建树闙T2DM模型。造模后第6 w腹腔注射STZ,在注射STZ前和注射后的每2 w,测定空腹血糖(FBG)、胰岛素(FINS)、C肽、胰岛素抵抗指数(HOMA-IR)、总胆固醇(TC)、甘油三酯(TG)、糖化血红蛋白(Hb A1c)、口服葡萄糖耐量试验(OGTT)等指标;自造模后的第8、16 w,观察胰腺、肝、肾脏的病理组织形态变化,检测血清TNF-α、IL-6表达水平。结果造模后,FBG持续升高,FINS、C肽先升高后下降,出现胰岛素抵抗;TG和TC在造模后持续升高,12 w后稳定在较高水平;肝、肾、胰腺器官表现为特征性的病理变化,且血清中TNF-α和IL-6增高。结论饲喂高糖、高脂饲料+腹腔注射STZ成功构建树闙T2DM模型,出现T2DM特征性病理变化,炎症细胞因子可能参与了T2DM的病理过程。
Objective To establish a tree model of type 2 diabetes mellitus (T2DM) for stem cell therapy and to analyze the related factors. Methods T2DM model was established by high glucose and high fat feeding combined with intraperitoneal injection of streptozotocin (STZ) 100 mg / kg. STZ was injected intraperitoneally on the 6th day after modeling, and the levels of fasting blood glucose (FBG), insulin (FINS), C peptide, insulin resistance index (HOMA-IR), total cholesterol ), Triglyceride (TG), Hb A1c and oral glucose tolerance test (OGTT) were measured. The histopathological changes of pancreas, liver and kidney were observed at the 8th and 16th weeks after modeling. Serum TNF-α, IL-6 expression levels. Results After modeling, FBG kept rising, FINS and C-peptide first increased and then decreased, and insulin resistance appeared. TG and TC increased continuously after modeling, and remained stable after 12 weeks. Liver, kidney and pancreas Manifested as characteristic pathological changes, and serum TNF-α and IL-6 increased. Conclusion T2DM model of tree 闙 was established successfully by feeding high-sucrose, high-fat diet + intraperitoneal injection of STZ. The characteristic pathological changes of T2DM were observed. Inflammatory cytokines may be involved in the pathological process of T2DM.