The enhanced sensitivity of a guided mode biosensor is analyzed by employing double-layered porous silicon grating structures. The grating-coupled waveguide structure consists of two porous silicon grating layers with different refractive indices. Simulations are carried out by changing the refractive index, which is due to the binding of biological molecules on the porous silicon pore can increase the refractive index of porous silicon. The numerical results show that this novel guided mode biosensor with a double-layered grating can provide not only a very high sensitivity but also a better reflectivity characteristic.